Tudyk is perhaps best known for his role as the playful, easy-going Hoban "Wash" Washburne in the television series Firefly by Joss Whedon. Although the series ran for only one season, Universal Studios bought the rights to the show and turned it into a film, Serenity, in which he reprised the role.
Among several guest spots on popular shows such as Arrested Development, he played a convicted pedophile on a popular episode of CSI: Crime Scene Investigation.
Most recently, Tudyk was cast as a special guest star in Joss Whedon's latest production, Dollhouse. The show features people who have had their personalities erased to be replaced by any fantasy a rich client can afford. Tudyk portrayed Alpha, a former "active" who accidentally downloads 48 separate personalities. Alpha serves as the main antagonist of the series' first season, with guest appearances in the show's second season.
Tudyk also guest starred in three episodes of ABC's modern remake of the television miniseries V.
Tudyk will provide the voice of superhero Green Arrow in the upcoming animated series Young Justice.